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41.
Julio Flores-Gonzalez Juan Carlos Cancino-Díaz Leslie Chavez-Galan 《International journal of molecular sciences》2020,21(22)
Recent advances in the field of flow cytometry (FCM) have highlighted the importance of incorporating it as a basic analysis tool in laboratories. FCM not only allows the identification of cell subpopulations by detecting the expression of molecules in the cell membrane or cytoplasm, but it can also quantify and identify soluble molecules. The proper functioning of the FCM requires six fundamental systems, from those related to the transport of events to the systems dedicated to the analysis of information. In this review, we have identified the main considerations that every FCM user must know for an optimal antibody panel design, the quality systems that must govern the FCM protocols to guarantee reproducible results in research or clinical laboratories. Finally, we have introduced the current evidence that highlights the relevance of FCM in the investigation and clinical diagnosis of respiratory diseases, establishing important advances in the basic and clinical study of diseases as old as Tuberculosis along with the recent proposals for the monitoring and classification of patients infected with the new SARS-CoV2 virus. 相似文献
42.
Surface wave breaking is a challenging two-phase flow process which plays an important role in numerous physical processes. A highly-turbulent unsteady breaking surge was investigated experimentally in a large facility, and substantial aeration occurred in the roller. The application of three optical flow techniques (Lucas-Kanade, Horn-Schunck and Farnback) to the air-water region was tested. The results indicated that the Farnback technique provided most accurate results, although some misleading results could be obtained near the air-water boundaries of the roller. The bore generation by a rapid gate closure showed a highly-unsteady complicated velocity field, with substantial free-surface deformations, wave breaking and formation of large coherent structures before the surge detached from the gate. Further upstream, the surge propagated as a hydraulic jump in translation and the data showed a marked shear region with a recirculation zone above, showing air-water flow features comparable to stationary hydraulic jumps. The upper and lower bounds of air-water flow region yielded data implying an air-to-water velocity ratio about 4–5 for a Froude number Fr1 = 2.1. 相似文献
43.
An innovate constant volume flow meter (ICVFM) using AAO leak element was developed, and then applied to measure the pumping speed of dry vacuum pumps. The AAO leak element was calibrated with dynamic differential pressure decay method. The results imply that the conductance of AAO leak is inversely proportional to the square root of the molecular mass, and remains unchanged with pressure varying from vacuum to atmosphere. Then the pumping speed was measured by a simple test system. In the end, the uncertainties of calibrating AAO leak element and measuring pumping speed were considered to confirm the measurement capability. The newly developed flow meter enables flow measurements at pressures ranging from vacuum through atmospheric pressure, which has potential application in the semiconductor industry. 相似文献
44.
The online measurement of wet gas with extremely-low liquid loading (Lockhart-Martinelli parameter lower than 0.02) remains a challenge. In this study, three types of throttle devices, Venturi, orifice plate and cone, are compared experimentally with air-water two-phase flow in a horizontal pipe of inner diameter of 50 mm. High-precision correlations are established to measure the gas and liquid flowrates via a single throttle device. Results show that the two-phase mass flow coefficient (K) of the three throttle devices all increase linearly with the liquid densiometric Froude number and the K correlations are established respectively to correct the gas mass flowrate deviation. The pressure loss ratio (δ) for Venturi is sensitive and monotonous to the liquid loading, which contributes to the high accuracy of liquid flowrate measurement. By incorporating the K correlations, both the gas and liquid mass flowrates can be predicted precisely. The relative error of the gas mass flowrate predicted by the Venturi is within ±2.0% at 95% confidence level, and that of the liquid mass flowrate is within ±15% at 90% confidence level. 相似文献
45.
为探究泵站压力管道的不同布置形式对管道振动的影响,选取宁夏盐环定泵站工程二泵站#1压力管道为研究对象,建立基于流固耦合的泵站压力管道水流ALGOR数值模型,并采用DASP振动测试系统获取振动信号对模拟结果进行验证,分析压力管道水流流态对管道振动的激励特征并提出优化设计方案。结果表明,压力管道进水管处、支管总管相接处及总出水管处均有强烈的振动,但进水管的振动较出水总管振动更为强烈;减振优化设计方案中,调整支管距离对降低水流对管道振动的激励效果微弱,增大管径及改变总管走向对其效果较好,其中总管走向在2°~5°之间较佳,不仅能降低管道振动,而且能减少泵站能耗损失。研究结果可为高扬程泵站压力管道的优化设计提供理论依据。 相似文献
46.
On the impingement heat transfer data,the experimental studies of air and liquid jets impingement to the flat surfaces were collected and critically reviewed.The oblique impingements of both single circular and planar slot jets were considered in particular.The review focused on the surface where the jet impingement cooling technique was utilized.The nozzle exit Reynolds numbers based on the hydraulic diameter varied in the range of 1,500–52,000.The oblique angles relative to the plane surface and the dimensionless jet-to-plate spacing vary in the range of 15°–90°and 2–12 respectively.The review suggested that the magnitude of maximum heat transfer shifted more for air jets compared with the liquid jets.The drop in the inclination angle and the jet-to-plate separation led to the increase in the asymmetry of heat transfer distribution.The displacement of maximum Nusselt number(heat transfer)locations was found to be sensitive to the inclination angle and the smaller jet-to-plate distance.Also,the Nusselt number correlations proposed by various researchers were discussed and compared with the results of the cited references. 相似文献
47.
环路热管作为一种高效的相变传热装置,其性能与位于蒸发器和储液槽之间的毛细芯结构密切相关。为了更深入研究双层毛细芯对环路热管传热性能的影响,利用不同颗粒直径铜粉制备双层毛细芯,在毛细芯总厚度为5 mm的条件下,通过调整大粒径和小粒径层的相对厚度来改变毛细芯厚度比,对平板型蒸发器环路热管启动和变工况运行进行实验测试。实验结果表明:在同一工况下,不同厚度比的双层毛细芯启动特性存在显著差异,启动过程中出现小粒径层蒸发效率低引起的温度过冲和环路热管中气液两相流变化导致的温度振荡;同时存在一个较优的双铜层毛细芯厚度比,大粒径(180~280μm)铜层厚度为3 mm可提高蒸发效率,小粒径(56~71μm)铜层厚度为2 mm可提供足够毛细抽吸力保证环路热管稳定运行。搭载该厚度毛细芯的环路热管不仅启动速度快(125 s),而且总热阻和蒸发器壁面温度均最低,最大加热功率达到120 W(21.10 W/cm~2),对应热阻为0.17 K/W。 相似文献
48.
《Advanced Powder Technology》2020,31(1):287-299
The parameters governing the crystallisation of paracetamol using various conventional techniques has been extensively studied, however the factors influencing the drug crystallisation using spray drying is not as well understood. The aim of this work was to investigate the crystallisation of an active pharmaceutical ingredient through evaporative crystallisation using a spray dryer to study the physicochemical properties of the drug and to use semi-empirical equations to gain insight into the morphology and particle size of the dried powder. Paracetamol solutions were spray dried at various inlet temperatures ranging from 60 °C to 120 °C and also from a series of inlet feed solvent compositions ranging from 50/50% v/v ethanol/water to 100% ethanol and solid-state characterisation was done. The size and morphology of the dried materials were altered with a change in spray drying parameters, with an increase in inlet temperature leading to an increase in particle Sauter mean diameter (from 3.0 to 4.4 µm) and a decrease in the particle size with an increase in ethanol concentration in the feed (from 4.6 to 4.4 µm) as a result of changes in particle density and atomised droplet size. The morphology of the dried particles consisted of agglomerates of individual crystallites bound together into larger semi-spherical agglomerates with a higher tendency for particles having crystalline ridges to form at higher ethanol concentrations of the feed. 相似文献
49.
A Takagi-Sugeno adaptive neuro-fuzzy inference system (TSFIS) model is developed and applied to a dataset of wellhead flow-test data for the Resalat oil field located offshore southern Iran, the objective is to assist in the prediction and control of multi-phase flow rates of oil and gas through the wellhead chokes. For this purpose, 182 test data points (Appendix 1) related to the Resalat field are evaluated. In order to predict production flow rate (QL) expressed as stock-tank barrels per day (STB/D), this dataset includes four selected input variables: upstream pressure (Pwh); wellhead choke sizes (D64); gas to liquid ratio (GLR); and, base solids and water including some water-soluble oil emulsion (BS&W). The test data points evaluated include a wide range of oil flow rate conditions and values for the four input variables recorded. The TSFIS algorithm applied involves five data processing steps: a) pre-processing, b) fuzzification, c) rules base and adaptive neuro-fuzzy inference engine, d) defuzzification, and e) post-processing of the fuzzy model. The developed TSFIS model for the Resalat oil field database predicted oil flow rate to a high degree of accuracy (root mean square error = 247 STB/D, correlation coefficient = 0.9987), which improves substantially on the commonly used empirical algorithms used for such predictions. TSFIS can potentially be applied in wellhead choke fuzzy controllers to stabilize flow in specific wells based on real-time input data records. 相似文献
50.
Yanzhao Luo Chenxi Ji Wenyuan He Yanqiang Liu Xiaoshan Yang Haibo Li 《Canadian Metallurgical Quarterly》2020,59(2):201-210
ABSTRACTThe character and cause of sliver defect on IF steel sheet surface are studied by means of SEM, unstable flow in mould could induce surface velocity and level fluctuations, leading to surface defects during continuous casting of steel. The nozzle clogging is a serious problem during the continuous casting of steel, due to its influence on the casting operations and products quality. In this study, the nail dipping method for measuring surface velocity and flow direction in molten steel were employed. The fluid flow in mould of whole casting sequence was investigated, especially during the nozzle clogging conditions. The results showed that when nozzle clogging occurred in the 7th heat, the flow velocities on R and L side of nozzle were 0.280 and 0.402?m/s, respectively. The surface defect ratio of hot-rolled and cold-rolled plates increases with the increase of heat flux deviation on both sides of the mould copper. The different clogging per cent on both sides of the nozzle will lead to asymmetry flow, the surface velocity is higher with the small clogging per cent side compared to that of relatively large per cent clogging side. 相似文献